Juve have lost two of their last five European home games, though, and they also suffered a shock domestic loss at the weekend as they went down 2-1 to lowly SPAL.
Here, Sports Mole looks at how Massimiliano Allegri could set out his side at the Allianz Stadium.
Both players are expected to start once again on Tuesday, joined in the side by the likes of Miralem Pjanic, Matuidi, Daniele Rugani and Ronaldo, who has scored in his last five Champions League games against Ajax, netting eight goals in total.
Matuidi hobbled off in the first leg, though, and his place in the side is therefore not guaranteed.
Chiellini is also likely to return from injury, which is also the case for Can, so changes from the first leg are likely to be minimal.