Dame Sarah Storey became Great Britain's most successful Paralympian after fighting back to take the 17th gold medal of her career.
Cycling star Storey powered home in the C4-5 road race to move past the 16-gold tally of former swimmer Mike Kenny, who she had matched on Tuesday by winning the C5 time trial.
On a glittering morning for Great Britain amidst the rain and gloom at the Fuji International Speedway, there was also a British one-two in the men's C1-3 event, with Benjamin Watson taking ahead of compatriot Fin Graham.
What's coming up on Thursday?
Rio champion Gordon Reid and runner-up Alfie Hewett will each hope to return to the men's wheelchair tennis singles final.
Discus thrower Dan Greaves seeks a sixth Paralympics medal, while taekwondo makes its maiden Games appearance, canoeing competition begins.
And there is another hectic schedule in the pool. Maisie Summers-Newton, who already has two golds at the Games, and Ellie Simmonds go in the Women's 400m Freestyle – S6 final, along with fellow Briton Grace Harvey.