England manager Roy Hodgson has confirmed that four players who were part of yesterday's squad for the Euro 2016 qualifier against Lithuania will not travel to Turin for Tuesday's friendly against Italy.
James Milner, Raheem Sterling and Danny Welbeck have all pulled out with slight injuries, while Leighton Baines returns to Everton as planned.
The latter's withdrawal sees Southampton full-back Ryan Bertrand called up to the squad, meaning that he could make his first appearance for his country since 2012.
Speaking to the Football Association's website, Hodgson said: "We knew we were going to be losing Raheem Sterling who had an injection last night on an ongoing injury and hopefully that will help him as we go forward.
"We have a problem with James Milner who had to leave, we decided to let Leighton Baines go home and of course we lost Danny Welbeck, who unfortunately picked up a slight knee injury [against Lithuania].
"So those four players won't be with us going forward but it means a chance for the other guys in the squad to show what they can do."
With Welbeck joining fellow forward Daniel Sturridge in pulling out, it leaves just captain Wayne Rooney and Harry Kane as the only two strikers available in Italy.
Welbeck scored the second in a 4-0 win over Lithuania to go clear as the leading scorer in Euro 2016 qualifying with six goals in five games.