Ronaldo underlined his value to his country by becoming the record goalscorer in European Championship history with 11 courtesy of a late brace in Tuesday's 3-0 success over Hungary.
The Portuguese dominated that Group F encounter in Budapest but only secured victory after striking three times in the final seven minutes.
Three-time tournament winners Germany will provide a much sterner test of the reigning champions' credentials on Saturday evening in Munich.
While Santos is delighted to have 36-year-old Juventus forward Ronaldo at his disposal, he is eager to ensure his entire squad remain ready to contribute.
"I have to motivate every single player, not just Ronaldo for instance. Portugal's value is based on our team," Santos told a press conference.
"It's great to have Ronaldo on our side, we're very proud to have him but Cristiano alone cannot win a match.
"For us, the next match is a great match and we will face it the same way we always do: we want to win, we believe we can win. This is a key game for us," said Santos.
"Germany is a great team but so is Portugal and we're going to display our best abilities on the pitch.
"Over the years, we have shown that we can play even when we don't have the ball and create chances and score and win, and that's what we're going to try to do."
Germany are in danger of a second successive early exit from a major tournament following a dismal display at the 2018 World Cup as defending champions.
Borussia Monchengladbach defender Matthias Ginter is determined to make his nation proud.
"Whenever we achieve success, it leads to such great euphoria across the country," he told EURO2020.com
"For this reason, it's firstly a responsibility we have, a duty to give our all."
Germany right-back Lukas Klostermann suffered a thigh injury in training and will be absent, while Portugal will be without defender Nuno Mendes due to a similar issue.