SM
| Venue: Weston Homes Community Stadium |
Colchester United | ||
| G | 1 | Dillon Barnes S  |
| CD-L | 5 | Luke Prosser S |
| CD-R | 6 | Frankie Kent |
| LB | 22 | Kane Vincent-Young |
| RB | 2 | Ryan Jackson |
| CM-L | 8 | Harry Pell |
| CM-R | 4 | Tom Lapslie |
| LM | 45 | Frank Nouble |
| RM | 7 | Courtney Senior S |
| F | 19 | Mikael Mandron S |
| RCF | 10 | Sammie Szmodics S |
| Sub | 11 | Brennan Dickenson |
| Sub | 9 | Luke Norris |
| Sub | 14 | Brandon Comley |
| Sub | 25 | Rene Gilmartin |
| Sub | 39 | Aaron Collins |
| Sub | 23 | Ryan Gondoh |
| Sub | 35 | Ollie Kensdale |
Macclesfield Town | ||
| G | 1 | Kieran O'Hara |
| CD-L | 27 | Nathan Cameron |
| CD-R | 5 | Fiacre Kelleher |
| LB | 3 | David Fitzpatrick |
| RB | 19 | James Pearson |
| CM-L | 24 | Michael Rose |
| CM-R | 23 | Danny Whitaker S |
| LM | 11 | Ryan Lloyd S |
| RM | 7 | Elliott Durrell S |
| F | 10 | Harry Smith |
| RCF | 15 | Ben Stephens |
| Sub | 20 | Peter Vincenti |
| Sub | 8 | Tyrone Marsh |
| Sub | 13 | Nathan Blissett |
| Sub | 6 | Jamie Grimes |
| Sub | 14 | Miles Welch-Hayes |
| Sub | 9 | Scott Wilson |
| Sub | 26 | Jordan Ponticelli |
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