Wolves, on the other hand, will make the trip to Villa Park with head coach Nuno Espirito Santo likely to make widespread changes to his squad given the club's current hectic schedule.
While Villa took their time to get up to speed after last year's promotion, Dean Smith will now be delighted with where his side currently find themselves after two-and-a-half months of the season.
Despite Saturday's 3-0 defeat at Manchester City ending a three-match unbeaten streak in the top flight, Villa now sit in 15th position, three points above the relegation zone.
The Birmingham-based outfit have also recorded two dominant wins over Crewe Alexandra and Brighton & Hove Albion in this competition, with Smith opting to make full use of his squad for each tie.
The same will likely occur on Wednesday evening, although Smith will recognise that the stakes are higher given the opposition and the possibility of securing another favourable tie in the next round.
As for Wolves, the EFL Cup is at their bottom of their priorities as they attempt to cope with the demands of European football, potentially through to the early months of 2020.
However, while there have been times when they have under-performed in recent outings, Nuno's side have managed to put together an eight-game unbeaten streak in all competitions.
Nuno will undoubtedly want to preserve that record ahead of Saturday's visit to Arsenal, although the Portuguese is unlikely to miss the opportunity to hand a rest to a number of key men, especially in light of Willy Boly's fractured ankle.
Nevertheless, the players who do feature in this derby clash will want to stake their claim for a regular spot, particularly any academy graduates who could be given the chance to break into the senior ranks on a regular basis.
Aston Villa form: LWDWWL
Wolverhampton Wanderers form: WWWDWD
Jack Grealish is a doubt for Villa having sustained a knock to his calf, although the playmaker will hope to be involved off the bench.
With Liverpool the visitors on Saturday, Smith should make wholesale alterations, with Kortney Hause coming into the team to face his former club.
Aston Villa possible starting lineup: Steer; Elmohamady, Konsa, Hause, Taylor; Hourihane, Luiz, Lansbury; El Ghazi, Trezeguet, Davis
Wolverhampton Wanderers possible starting lineup: Ruddy; Vallejo, Coady, Kilman; Jonny, Dendoncker, Gibbs-White, Vinagre; Neto, Cutrone, Jota
We say: Aston Villa 3-1 Wolverhampton Wanderers
While both teams will make alterations, it is difficult to back against Villa making it through to the next round. Wolves will be keen to get one over their rivals, although Villa's probable greater experience may prove to be decisive.