The campaign aims to tackle touchline behaviour at under-18s matches by encouraging parents to sign up to the pledge.
Kane and Houghton have written a letter to parents and other spectators, which was painted around the side of the pitch at their respective childhood clubs – Ridgeway Rovers and Boldon Girls.
Houghton added: "Positivity is essential in football. It's important to create an environment where everyone enjoys playing.
"There are small things you can do on the touchline – like applauding good play from both teams – that has a big impact on young players."
The FA's Respect campaign was relaunched in the 2018-19 season having originally being introduced 10 years earlier, with the new mantra being 'We Only Do Positive'.