British number one Katie Boulter was eliminated from the Australian Open courtesy of a three-set defeat to Veronika Kudermetova, denying her nation a Grand Slam best in the process.
The 22nd seed fought back against the former world number nine after losing a tight first set, but her revival fell short as Kudermetova prevailed 7-6[3] 2-6 6-2 to reach the third round.
The Russian will now face Brazilian 15th seed Beatriz Haddad Maia for a place in the fourth round, and Boulter's defeat means that GB have fallen short of setting a new national record Down Under.
Had Boulter prevailed on Thursday, Great Britain would have had four players in the last 32 of the Australian Open for the first time in the Open Era, as Jack Draper and Jacob Fearnley are both in the third round of the men's singles.
The duo's second-round successes earlier this week preceded a momentous win for Emma Raducanu, who has made it to the third round Down Under for the first time in her career thanks to her battling win over Amanda Anisimova.
The former US Open champion triumphed 6-3 7-5 over the American, but the scoreline did not tell the full story for Raducanu, who found herself a break down in each set but managed to turn the tide.
Each of Raducanu's first three appearances at the Australian Open had ended in the second round, but the 22-year-old has finally banished that curse and will be rewarded with a daunting match against Iga Swiatek in round three.
Daniil Medvedev shocked by Learner Tien in five-set marathon
Elsewhere on Thursday, the action did not wrap up until close to 3am in the morning local time, as former US Open champion Daniil Medvedev was stunned in a five-set marathon with American starlet Learner Tien.
Medvedev almost pulled off an exceptional comeback against the 19-year-old - who had to come through qualifying to make it through to the main draw - but Tien dug ridiculously deep in a final-set tie-breaker to win an epic battle 6-3 7-6[4] 6-7[8] 1-6 7-6[7].
Fifth seed Medvedev is now the highest-ranked player to fall in the men's tournament so far, while Tien is the only qualifier to make it through to the third round of the men's draw - he faces France's Corentin Moutet next up.
However, defending champion Jannik Sinner did not suffer the same fate as Medvedev, surviving an early scare against Australian wildcard Tristan Schoolkate as he took down the home favourite 4-6 6-4 6-1 6-3.
Holger Rune, Alex de Minaur, Iga Swiatek, Jasmine Paolini and Elena Rybakina also advanced to round three, but Frances Tiafoe was upset by Fabian Marozsan, and another shock result in the men's section saw Hubert Hurkacz knocked out in straight sets by Miomir Kecmanovic. body check tags ::