Arsene Wenger has made three changes to the side beaten by Bradford City in the Capital One Cup last week.
Mikel Arteta, Theo Walcott and Alex Oxlade-Chamberlain come into the starting lineup for the Gunners, while Gervinho misses out.
Olivier Giroud is also back in the squad, but starts on the bench.
Pavel Pogrebnyak returns from injury for Reading, following their 3-0 defeat by Sunderland last Tuesday.
Noel Hunt keeps his place in the starting XI, but Adam Le Fondre drops to the bench.
Reading: Federici, Cummings, Shorey, Gorkss, Mariappa, Leigertwood, Tabb, Kebe, McAnuff, Hunt, Pogrebnyak
Arsenal: Szczesny, Sagna, Gibbs, Mertesacker, Vermaelen, Arteta, Wilshere, Cazorla, Chamberlain, Podolski, Walcott