Granada will be without the services of on-loan Liverpool defender Tiago Ilori, forward Piti and goalkeeper Roberto for their trip to Atletico Madrid on Wednesday evening.
Ilori and Piti were both present for first-team training on Monday, but did not take part in the full session, effectively ruling them out of the clash in the capital.
Roberto, meanwhile, picked up an ankle knock in the 1-0 win over Elche at the weekend and is expected to be absent at the Calderon.
Granada have won 10 of their 29 league games this season, with a total of 34 points leaving them in 11th position in the table.