SM
| Venue: Proact Stadium |
Chesterfield | ||
| G | 1 | Jake Eastwood |
| CD-L | 4 | Sam Hird |
| CD-R | 5 | Scott Wiseman |
| LB | 3 | Jerome Binnom-Williams |
| RB | 26 | Jak McCourt |
| AM | 17 | Connor Dimaio |
| LM | 25 | Louis Reed |
| RM | 28 | Robbie Weir |
| F | 9 | Kristian Dennis |
| AM-L | 24 | Andy Kellett |
| AM-R | 15 | Joe Rowley |
| Sub | 10 | Chris O'Grady |
| Sub | 19 | Diego De Girolamo |
| Sub | 7 | Reece Mitchell |
| Sub | 31 | Ify Ofoegbu |
| Sub | 29 | Ricky German |
| Sub | 16 | Charlie Wakefield |
| Sub | 30 | Dylan Parkin |
Colchester United | ||
| G | 1 | Sam Walker |
| CD-L | 6 | Frankie Kent |
| CD-R | 18 | Tom Eastman |
| LB | 22 | Kane Vincent-Young |
| RB | 2 | Ryan Jackson |
| AM | 10 | Sammie Szmodics |
| LM | 14 | Brandon Comley |
| RM | 4 | Tom Lapslie |
| F | 19 | Mikael Mandron |
| AM-L | 17 | Kyel Reid |
| AM-R | 7 | Drey Wright |
| Sub | 16 | Sean Murray |
| Sub | 28 | Kurtis Guthrie |
| Sub | 29 | Rene Gilmartin |
| Sub | 24 | Craig Slater |
| Sub | 12 | Cole Kpekawa |
| Sub | 27 | Oluwasanmi Odelusi |
| Sub | 21 | Brandon Hanlan |
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