SM
| Venue: Weston Homes Community Stadium |
Colchester United | ||
| G | 25 | Rene Gilmartin |
| CD-L | 5 | Luke Prosser |
| CD-R | 6 | Frankie Kent |
| LB | 22 | Kane Vincent-Young |
| RB | 2 | Ryan Jackson |
| AM | 45 | Frank Nouble |
| LM | 24 | Ben Stevenson |
| RM | 8 | Harry Pell |
| F | 39 | Abobaker Eisa S  |
| AM-L | 10 | Sammie Szmodics |
| AM-R | 7 | Courtney Senior |
| Sub | 11 | Brennan Dickenson |
| Sub | 15 | Callum Roberts |
| Sub | 18 | Tom Eastman |
| Sub | 9 | Luke Norris |
| Sub | 1 | Dillon Barnes |
Cheltenham Town | ||
| G | 1 | Scott Flinders |
| CD | 4 | Ben Tozer |
| CD-L | 23 | Jordan Forster |
| CD-R | 24 | Charlie Raglan |
| CM-L | 25 | Jordan Tillson |
| CM-R | 26 | Chris Clements S |
| AM | 8 | Kevin Dawson |
| LM | 3 | Chris Hussey |
| RM | 11 | Ryan Broom S |
| CF-L | 27 | Rakish Bingham S |
| CF-R | 32 | Luke Varney |
| Sub | 20 | Jacob Maddox |
| Sub | 29 | Tyrone Barnett |
| Sub | 21 | Billy Waters |
| Sub | 7 | Conor Thomas |
| Sub | 22 | Rhys Lovett |
| Sub | 9 | Immanuelson Duku |
| Sub | 28 | Cameron Pring |
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