SM
| Venue: Weston Homes Community Stadium |
Colchester United | ||
| G | 1 | Sam Walker |
| CD | 26 | Ryan Inniss S  |
| CD-L | 6 | Frankie Kent |
| CD-R | 18 | Tom Eastman |
| CM-L | 16 | Sean Murray |
| CM-R | 4 | Tom Lapslie |
| LM | 22 | Kane Vincent-Young |
| RM | 2 | Ryan Jackson |
| F | 28 | Kurtis Guthrie S |
| CF-L | 7 | Drey Wright |
| CF-R | 10 | Sammie Szmodics S |
| Sub | 20 | Courtney Senior |
| Sub | 19 | Mikael Mandron |
| Sub | 27 | Junior Ogedi-Uzokwe |
| Sub | 29 | Rene Gilmartin |
| Sub | 5 | Luke Prosser |
| Sub | 14 | Brandon Comley |
| Sub | 12 | Cole Kpekawa |
Grimsby Town | ||
| G | 33 | Jake Kean |
| CD | 14 | Karleigh Osborne |
| CD-L | 6 | Danny Collins |
| CD-R | 5 | Nathan Clarke |
| LB | 3 | Paul Dixon S |
| RB | 2 | Ben Davies |
| CM | 8 | Mitchell Rose |
| CM-L | 19 | Luke Summerfield |
| CM-R | 7 | James Berrett |
| CF-L | 29 | Jamille Matt S |
| CF-R | 34 | Charles Vernam S |
| Sub | 25 | Martyn Woolford |
| Sub | 21 | Scott Vernon |
| Sub | 9 | JJ Hooper |
| Sub | 17 | Harry Cardwell |
| Sub | 12 | Zak Mills |
| Sub | 30 | Ben Killip |
| Sub | 20 | Diallang Jaiyesimi |
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