SM
| Venue: Weston Homes Community Stadium |
Colchester United | ||
| G | 1 | Dillon Barnes S  |
| CD-L | 6 | Frankie Kent |
| CD-R | 18 | Tom Eastman |
| LB | 22 | Kane Vincent-Young |
| RB | 2 | Ryan Jackson |
| AM | 10 | Sammie Szmodics S |
| LM | 36 | Sam Saunders |
| RM | 4 | Tom Lapslie |
| F | 45 | Frank Nouble |
| AM-L | 11 | Brennan Dickenson S |
| AM-R | 7 | Courtney Senior S |
| Sub | 24 | Ben Stevenson |
| Sub | 19 | Mikael Mandron |
| Sub | 39 | Abobaker Eisa |
| Sub | 9 | Luke Norris |
| Sub | 29 | Ethan Ross |
| Sub | 35 | Ollie Kensdale |
| Sub | 27 | Noah Chilvers |
Grimsby Town | ||
| G | 1 | James McKeown |
| CD-L | 6 | Danny Collins |
| CD-R | 24 | Harry Davis S |
| LB | 2 | Reece Hall-Johnson S |
| RB | 27 | Luke Hendrie |
| CM-L | 16 | Martyn Woolford S |
| CM-R | 7 | Jake Hessenthaler |
| LM | 14 | Joe Grayson S |
| RM | 18 | Charles Vernam S |
| CF-L | 8 | Kristian Dennis |
| CF-R | 17 | Harry Cardwell |
| Sub | 29 | Rumarn Burrell |
| Sub | 15 | Harry Clifton |
| Sub | 21 | Ahkeem Rose |
| Sub | 23 | Sam Ian Russell |
| Sub | 25 | Alex Whitmore |
| Sub | 31 | Matthew Pollock |
| Sub | 30 | Jock Curran |
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