SM
| Venue: Weston Homes Community Stadium |
Colchester United | ||
| G | 1 | Dillon Barnes S  |
| CD-L | 6 | Luke Prosser S |
| CD-R | 5 | Frankie Kent |
| LB | 22 | Kane Vincent-Young S |
| RB | 2 | Ryan Jackson |
| CM-L | 8 | Harry Pell |
| CM-R | 4 | Tom Lapslie |
| LM | 11 | Brennan Dickenson |
| RM | 7 | Courtney Senior S |
| F | 45 | Frank Nouble S |
| RCF | 10 | Sammie Szmodics |
| Sub | 39 | Aaron Collins |
| Sub | 14 | Brandon Comley |
| Sub | 19 | Mikael Mandron |
| Sub | 18 | Tom Eastman |
| Sub | 9 | Luke Norris |
| Sub | 29 | Ethan Ross |
| Sub | 16 | Diaz Wright |
Port Vale | ||
| G | 1 | Scott Brown |
| CD-L | 13 | Connell Rawlinson |
| CD-R | 15 | Nathan Smith |
| LB | 3 | Cristian Montano |
| RB | 2 | James Gibbons S |
| CM-L | 4 | Luke Joyce |
| CM-R | 8 | Emmanuel Oyeleke |
| LM | 11 | Luke Hannant S |
| RM | 7 | David Worrall |
| CF-L | 10 | Ricky Miller |
| CF-R | 9 | Tom Pope S |
| Sub | 5 | Leon Legge |
| Sub | 14 | Scott Quigley |
| Sub | 19 | Louis Dodds |
| Sub | 18 | Michael Tonge |
| Sub | 12 | Sam Hornby |
| Sub | 21 | Theo Vassell |
| Sub | 17 | Idris Kanu |
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