SM
| Venue: Sincil Bank |
Lincoln City | ||
| G | 18 | Matthew Gilks |
| CD-L | 16 | Michael Bostwick |
| CD-R | 24 | Cian Bolger |
| LB | 14 | Harry Toffolo |
| RB | 23 | Neil Eardley |
| CM-L | 6 | Mark O'Hara |
| CM-R | 4 | Michael O'Connor S  |
| LM | 11 | Bruno Andrade S |
| RM | 19 | Danny M. Rowe |
| CF-L | 29 | John Akinde |
| CF-R | 9 | Matthew Rhead S |
| Sub | 7 | Tom Pett |
| Sub | 26 | Harry Anderson |
| Sub | 17 | Shay McCartan |
| Sub | 25 | Jordan Roberts |
| Sub | 15 | James Wilson |
| Sub | 1 | Josh Vickers |
Tranmere Rovers | ||
| G | 1 | Scott Davieson |
| CD-L | 6 | Emmanuel Monthe |
| CD-R | 4 | Sid Nelson |
| LB | 24 | Zoumana Bakayogo |
| RB | 14 | Jake Caprice |
| CM-L | 8 | Jay Harris |
| CM-R | 28 | Oliver Banks |
| LM | 11 | Connor Jennings |
| RM | 19 | Kieron Morris |
| CF-L | 9 | Paul Mullin S |
| CF-R | 10 | James Norwood |
| Sub | 22 | Harvey Gilmour |
| Sub | 29 | Chris Dagnall |
| Sub | 27 | Ben Pringle |
| Sub | 25 | Luke Pilling |
| Sub | 18 | Jon Smith |
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