SM
| Venue: Vale Park |
Port Vale | ||
| G | 1 | Robert Lainton |
| CD | 6 | Antony Kay S  |
| CD-L | 3 | Zak Jules |
| CD-R | 24 | Nathan Smith |
| CM-L | 34 | Luke Hannant |
| CM-R | 32 | Michael Tonge |
| AM | 10 | David Worrall |
| LM | 11 | Cristian Montano S |
| RM | 21 | James Gibbons |
| CF-L | 28 | Donovan Wilson S |
| CF-R | 9 | Tom Pope |
| Sub | 4 | Thomas Anderson |
| Sub | 31 | Ben Whitfield |
| Sub | 15 | Anton Forrester |
| Sub | 8 | Danny Pugh |
| Sub | 14 | Tyrone Barnett |
| Sub | 7 | Marcus Myers-Harness |
| Sub | 23 | Ryan Boot |
Colchester United | ||
| G | 1 | Sam Walker |
| CD-L | 18 | Tom Eastman |
| CD-R | 6 | Frankie Kent |
| LB | 22 | Kane Vincent-Young |
| RB | 2 | Ryan Jackson |
| AM | 7 | Drey Wright S |
| LM | 16 | Sean Murray |
| RM | 4 | Tom Lapslie |
| F | 19 | Mikael Mandron S |
| AM-L | 20 | Courtney Senior S |
| AM-R | 10 | Sammie Szmodics |
| Sub | 5 | Luke Prosser |
| Sub | 28 | Kurtis Guthrie |
| Sub | 14 | Brandon Comley |
| Sub | 29 | Rene Gilmartin |
| Sub | 12 | Cole Kpekawa |
| Sub | 31 | Tariq Issa |
| Sub | 27 | Junior Ogedi-Uzokwe |
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