SM
| Venue: Sands Venue Stadium |
Scunthorpe United | ||
| G | 25 | Jake Eastwood |
| CD-L | 23 | Rory McArdle |
| CD-R | 26 | Harrison McGahey |
| LB | 3 | Kgosietsile Ntlhe |
| RB | 2 | Jordan Clarke |
| CM-L | 5 | Yann Songo'o |
| CM-R | 14 | James Perch S  |
| LM | 30 | Abobaker Eisa |
| RM | 20 | Alex Gilliead |
| CF-L | 17 | Lee Novak |
| CF-R | 10 | Kevin Van Veen |
| Sub | 45 | John Mcatee |
| Sub | 6 | Andy Butler |
| Sub | 13 | Jamie Ward |
| Sub | 28 | Junior Brown |
| Sub | 33 | Jamie Proctor |
| Sub | 12 | Andy Dales |
| Sub | 1 | Rory Watson |
Colchester United | ||
| G | 1 | Dean Gerken |
| CD-L | 5 | Luke Prosser S |
| CD-R | 18 | Tom Eastman |
| LB | 3 | Cohen Bramall |
| RB | 2 | Ryan Jackson |
| AM | 49 | Kwame Poku S |
| LM | 8 | Harry Pell |
| RM | 14 | Brandon Comley |
| F | 9 | Luke Norris S |
| AM-L | 45 | Frank Nouble |
| AM-R | 7 | Courtney Senior S |
| Sub | 13 | Theo Robinson |
| Sub | 15 | Callum Harriott |
| Sub | 4 | Tom Lapslie |
| Sub | 6 | Omar Sowunmi |
| Sub | 26 | Luke Gambin |
| Sub | 29 | Ethan Ross |
| Sub | 21 | Ryan Clampin |
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