SM
| Venue: Prenton Park |
Tranmere Rovers | ||
| G | 1 | Scott Davieson |
| CD-L | 4 | Ritchie Sutton S  |
| CD-R | 5 | Steve McNulty |
| LB | 24 | Zoumana Bakayogo |
| RB | 14 | Jake Caprice |
| CM-L | 28 | Oliver Banks |
| CM-R | 8 | Jay Harris |
| LM | 11 | Connor Jennings |
| RM | 18 | Jon Smith S |
| CF-L | 20 | Cole Stockton S |
| CF-R | 10 | James Norwood |
| Sub | 6 | Emmanuel Monthe |
| Sub | 7 | Ben Tollitt |
| Sub | 22 | Harvey Gilmour |
| Sub | 9 | Paul Mullin |
| Sub | 16 | Mark Ellis |
| Sub | 29 | Larnell Cole |
| Sub | 25 | Luke Pilling |
| Sub | 23 | Shamal George |
Cheltenham Town | ||
| G | 1 | Scott Flinders |
| CD-L | 15 | William Boyle |
| CD-R | 5 | Johnny Mullins |
| LB | 17 | Joshua Debayo S |
| RB | 21 | Aden Baldwin S |
| CM-L | 6 | Nigel Atangana |
| CM-R | 4 | Ben Tozer |
| LM | 20 | Jacob Maddox S |
| RM | 8 | Kevin Dawson S |
| CF-L | 10 | Liam McAlinden S |
| CF-R | 9 | Immanuelson Duku S |
| Sub | 11 | Ryan Broom |
| Sub | 16 | Alex Addai |
| Sub | 24 | Kalvin Kalala |
| Sub | 7 | Conor Thomas |
| Sub | 23 | Jordan Forster |
| Sub | 14 | Tom Smith |
| Sub | 22 | Rhys Lovett |
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