SM
| Venue: Prenton Park |
Tranmere Rovers | ||
| G | 13 | Joe Murphy |
| D | 26 | Peter Clarke |
| M | 3 | Liam Ridehalgh |
| D | 21 | George Ray |
| D | 2 | Lee O'Connor |
| M | 8 | Jay Spearing |
| M | 19 | Liam Feeney |
| M | 22 | Paul Lewis |
| M | 17 | Otis Khan |
| A | 11 | Corey Taylor |
| A | 28 | David Nugent |
Colchester United | ||
| G | 29 | Shamal George |
| D | 5 | Tommy Smith |
| D | 18 | Tom Eastman |
| D | 2 | Miles Welch-Hayes |
| D | 21 | Ryan Clampin |
| M | 4 | Tom Lapslie |
| M | 22 | Brendan Sarpong-Wiredu |
| M | 14 | Noah Chilvers |
| M | 23 | Kwame Poku |
| A | 45 | Frank Nouble |
| A | 39 | Michael Folivi |
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