Prince Ali's request to postpone Friday's FIFA presidential election has been rejected by the Court of Arbitration for Sport (CAS).
The Jordanian, who is one of five candidates hoping to replace outgoing president Sepp Blatter, lodged a formal request to CAS on Tuesday.
The 40-year-old argued for transparent voting booths to be used, and after FIFA's election commission turned down his idea, he went to CAS.
Prince Ali has been unsuccessful again, though, and Friday's vote will go ahead in Zurich as planned.
A statement from Prince Ali read: "My central point throughout the campaign has been the honesty and integrity of the election, principles worth fighting for. I advocated for transparent voting booths on behalf of FA Presidents who want to vote their conscience, without worrying that someone with a different agenda is looking over their shoulder.
"I fought for them at FIFA and at CAS, and brought transparent booths to Zurich to eliminate any excuse for not using them. I have done all I can. I regret that the system let us down. The only positive aspect of today's ruling is it that the election will now go forward as planned, and the media will be closely watching for any evidence that anyone is photographing their ballot.
"It is now imperative that voters abide by the ban on mobile phones and cameras in the voting booth. I look forward to Friday's vote and remain as committed as ever to the goal of reforming FIFA."
Gianni Infantino, Sheikh Salman, Tokyo Sexwale and Jerome Champagne are the other candidates in the vote.