Olympic champion Missy Franklin failed to medal in the 100m backstroke final at the World Championships in Kazan after finishing fifth.
The 20-year-old, who won Olympic gold in this event at London 2012, fell behind the leaders in the first 50m following a poor start.
The nine-time world champion failed to recover and Australian duo Emily Seebohm and Madison Wilson were able to take the reins and claim gold and silver respectively.
Seebohm clocked a winning time of 58.26s, while Franklin touched the wall in 59.40s, two places behind bronze-medal winner Mie Oe Nielsen of Denmark.
Meanwhile, Great Britain's Lauren Quigley finished in seventh place.