Great Britain's Laura Robson has failed to reach the first round of the French Open after a defeat to Karolina Pliskova in a third qualifying round tie.
Despite winning the first set, the 19-year-old went down 6-4 5-7 4-6 to her Czech opponent.
Elena Baltacha, Anne Keothavong and Heather Watson will be Britain's female representatives at Roland Garros.
The second Grand Slam of the year starts on Sunday.