The last-16 tie is delicately poised following a 1-1 draw in the first leg back in February, and it promises to be a fascinating battle to reach the final eight of the competition.
Barca are bidding to win the Champions League for the first time since 2015 and will meet either Bayern Munich or Chelsea in the quarter-finals should they advance this weekend.
Here, Sports Mole looks at the home side could line up at Camp Nou.
Barca will be without the services of both Sergio Busquets and Arturo Vidal through suspension, meaning that Quique Setien has decisions to make when it comes to the middle of the park.
Frenkie de Jong has overcome his injury problems and is a certain starter, while it seems likely that Ivan Rakitic and Sergi Roberto will also feature in midfield, allowing Nelson Semedo to keep his spot at right-back.
Samuel Umtiti is still out with a knee problem, but Clement Lenglet has overcome a recent issue, while Antoine Griezmann is also back from the thigh complaint that saw him miss the end of the La Liga campaign.
Ousmane Dembele trained on Wednesday as he continues to recover from a long-term hamstring problem, but it seems that this match will come too soon for the France international.
Luis Suarez was absent from the first leg against Napoli through injury, but the Uruguay international is available for the return fixture and should start alongside Lionel Messi and Griezmann in attack.