The Old Lady are currently second in the section and have already booked their spot in the knockout round of the competition ahead of the clash at Camp Nou.
Andrea Pirlo's side can still top the group, although they will have to beat Barca by three goals or more, or by any two-goal margin other than 2-0, which was the result in the reverse game in Turin.
Here, Sports Mole looks at how Juve could line up in Spain.
Juve will again be unable to call upon defensive duo Merih Demiral or Giorgio Chiellini against Barca, while Gianluigi Buffon is on the sidelines with a thigh problem.
Pirlo otherwise has a strong squad to choose from, though, and like Barca boss Ronald Koeman, the Italian is expected to resist the temptation to make wholesale changes to his first-choice XI.
Ronaldo missed out on the chance to face Barca in the reverse game in Turin due to coronavirus, but the Portugal international is expected to be in the starting XI for this game.