Barca will attempt to hold onto first spot in the section when they welcome Andrea Pirlo's side, who have also already advanced to the next round ahead of their final group-stage fixture.
Here, Sports Mole looks at how the home side could line up in the European Cup.
Barca will again be without the services of Sergi Roberto, Gerard Pique and Ansu Fati on Tuesday, while the Catalan giants confirmed on Sunday that Ousmane Dembele has suffered another hamstring problem.
Dembele picked up the injury during the second half of Saturday's clash with Cadiz and is now facing another extended period on the sidelines in what is a huge blow for the club and the player himself.
Samuel Umtiti is again a major doubt due to ongoing knee problems, but Ronald Araujo has recovered from injury and could come into the starting XI against the Italian champions.
Pjanic could replace Sergio Busquets in midfield as the latter is only just back from a knee problem, while Oscar Mingueza is likely to be the player to drop out if Araujo, who was on the bench on Saturday, is handed a start.