The 33-year-old was unable to take part in Barcelona's training session on Thursday, with a calf problem seeing the Spaniard complete "specific work" away from the main group, the club confirmed.

Sunday will prove to be Iniesta's final chance to play in El Clasico as the midfielder prepares to leave Camp Nou at the end of the season following almost 22 years with the club.

Iniesta is still hopeful of being fit for the clash against Los Blancos, according to a number of reports in Spain, but his absence from Thursday's training session has caused concern.

Barcelona wrapped up the La Liga title by beating Deportivo La Coruna last weekend, while Real Madrid are currently battling Atletico Madrid for second position in the table.

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Premier League Table

	Team	P	W	D	L	F	A	GD	PTS
1	Barcelona	13	11	0	2	40	12	28	33
2	Real Madrid	12	8	3	1	25	11	14	27
3	Atletico Madrid Atletico	13	7	5	1	19	7	12	26
4	Villarreal	12	7	3	2	23	19	4	24
5	Osasuna	13	6	3	4	17	20	-3	21
6	Athletic Bilbao	13	5	5	3	19	13	6	20
7	Real Betis Betis	13	5	5	3	14	12	2	20
8	Real Sociedad	13	5	3	5	11	10	1	18
9	Mallorca	13	5	3	5	10	10	0	18
10	Girona Girona	13	5	3	5	16	17	-1	18
11	Celta Vigo	13	5	2	6	20	22	-2	17
12	Rayo Vallecano	12	4	4	4	13	13	0	16
13	Sevilla	13	4	3	6	12	18	-6	15
14	Leganes	13	3	5	5	13	16	-3	14
15	Alaves Alaves	13	4	1	8	14	22	-8	13
16	Las Palmas Las Palmas	13	3	3	7	16	22	-6	12
17	Getafe	13	1	7	5	8	11	-3	10
18	Espanyol	12	3	1	8	11	22	-11	10
19	Real Valladolid Valladolid	13	2	3	8	10	25	-15	9
20	Valencia	11	1	4	6	8	17	-9	7