The Three Lions welcomed Harry Maguire back to the bench for their 0-0 draw with Scotland, and the Manchester United man could find himself back in the team alongside John Stones, which would see Tyrone Mings harshly dropped.
Kyle Walker was left out entirely on Friday but could force his way back into the side over Reece James, but Luke Shaw should keep his place on the left over Ben Chilwell after the Chelsea man came into close contact with the COVID-positive Billy Gilmour.
Jordan Henderson was also an unused substitute last time out and is in consideration for a start this week, but Southgate may elect to keep faith in Declan Rice and Kalvin Phillips for their final group game.
The final third is where Southgate's real dilemmas lie, as the England manager has witnessed some of his attacking talents flatter to deceive on the big stage so far, and changes will surely be afoot.
Mason Mount will also miss out through self-isolation, so Jack Grealish - assuming he can shake off some discomfort - must be a strong contender to start this game as Phil Foden is also handed another chance.
Raheem Sterling has fond memories of playing the Czech Republic after scoring three of England's five goals against them in a March 2019 victory, but he is another player whose spot is at risk as Jadon Sancho hopes to finally make his Euros debut.
Harry Kane is failing to fire in an England shirt and failed to complete the 90 minutes against either Croatia or Scotland, but Southgate is unlikely to consider dropping his talisman.
England possible starting lineup: Pickford; Walker, Stones, Maguire, Shaw; Rice, Phillips; Sancho, Foden, Grealish; Kane
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