SM
| Venue: Weston Homes Community Stadium |
Colchester United | ||
| G | 1 | Sam Walker |
| CD | 6 | Frankie Kent |
| CD-L | 12 | Cole Kpekawa |
| CD-R | 18 | Tom Eastman |
| CM-L | 16 | Sean Murray S  |
| CM-R | 4 | Tom Lapslie |
| LM | 22 | Kane Vincent-Young |
| RM | 2 | Ryan Jackson |
| F | 19 | Mikael Mandron S |
| CF-L | 10 | Sammie Szmodics |
| CF-R | 7 | Drey Wright S |
| Sub | 14 | Brandon Comley |
| Sub | 21 | Brandon Hanlan |
| Sub | 28 | Kurtis Guthrie |
| Sub | 29 | Rene Gilmartin |
| Sub | 17 | Kyel Reid |
| Sub | 24 | Craig Slater |
| Sub | 35 | Cameron James |
Cambridge United | ||
| G | 1 | David Forde |
| CD-L | 12 | George Taft S |
| CD-R | 6 | Leon Legge |
| LB | 3 | Jake Carroll S |
| RB | 2 | Bradley Halliday |
| AM | 20 | Jeavani Brown |
| LM | 5 | Greg Taylor |
| RM | 4 | Gary Deegan |
| F | 9 | Uche Ikpeazu |
| AM-L | 19 | David Amoo S |
| AM-R | 18 | George Maris |
| Sub | 10 | Adebayo Azeez |
| Sub | 11 | Harrison Dunk |
| Sub | 21 | Medy Elito |
| Sub | 16 | Liam O'Neil |
| Sub | 13 | Dimitar Mitov |
| Sub | 15 | Emma Osaoabe |
| Sub | 24 | Leon Davies |
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