SM
| Venue: Weston Homes Community Stadium |
Colchester United | ||
| G | 1 | Sam Walker |
| CD-L | 6 | Frankie Kent |
| CD-R | 18 | Tom Eastman |
| LB | 22 | Kane Vincent-Young |
| RB | 2 | Ryan Jackson |
| AM | 10 | Sammie Szmodics S  |
| LM | 14 | Brandon Comley |
| RM | 4 | Tom Lapslie |
| F | 19 | Mikael Mandron |
| AM-L | 17 | Kyel Reid S |
| AM-R | 7 | Drey Wright S |
| Sub | 21 | Brandon Hanlan |
| Sub | 35 | Cameron James |
| Sub | 16 | Sean Murray |
| Sub | 29 | Rene Gilmartin |
| Sub | 24 | Craig Slater |
| Sub | 28 | Kurtis Guthrie |
| Sub | 27 | Oluwasanmi Odelusi |
Port Vale | ||
| G | 23 | Ryan Boot |
| CD-L | 33 | Gavin Gunning |
| CD-R | 24 | Nathan Smith |
| DM | 6 | Antony Kay |
| LB | 8 | Danny Pugh |
| RB | 5 | Joe Davis |
| CM-L | 7 | Marcus Myers-Harness |
| CM-R | 31 | Ben Whitfield S |
| LM | 11 | Cristian Montano S |
| RM | 10 | David Worrall |
| F | 9 | Tom Pope |
| Sub | 3 | Tyler Denton |
| Sub | 14 | Tyrone Barnett |
| Sub | 32 | Michael Tonge |
| Sub | 19 | Dan Turner |
| Sub | 20 | Graham Kelly |
| Sub | 1 | Robert Lainton |
| Sub | 15 | Anton Forrester |
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