SM
| Venue: Prenton Park |
Tranmere Rovers | ||
| G | 1 | Ross Doohan |
| D | 19 | Neill Byrne |
| D | 14 | Jordan Turnbull |
| M | 4 | Lee O'Connor |
| D | 2 | Josh Dacres-Cogley |
| D | 3 | Ethan Bristow |
| M | 16 | Jon Nolan |
| M | 11 | Josh Hawkes |
| M | 8 | Reece McAlear |
| A | 10 | Kane Hemmings |
| A | 20 | Elliott Nevitt |
Colchester United | ||
| G | 13 | Kieran O'Hara |
| D | 4 | Luke Chambers |
| D | 6 | Tom Dallison |
| D | 3 | Ryan Clampin |
| D | 22 | Junior Tchamadeu |
| M | 8 | Cole Skuse |
| M | 19 | Alex Newby |
| M | 14 | Noah Chilvers |
| M | 33 | Marley Marshall |
| A | 11 | Freddie Sears |
| M | 45 | Frank Nouble |
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