SM
| Venue: Prenton Park |
Tranmere Rovers | ||
| G | 1 | Scott Davieson |
| CD-L | 6 | Emmanuel Monthe |
| CD-R | 5 | Steve McNulty |
| LB | 24 | Zoumana Bakayogo |
| RB | 14 | Jake Caprice |
| CM-L | 28 | Oliver Banks |
| CM-R | 12 | Luke McCullough |
| LM | 11 | Connor Jennings |
| RM | 18 | Jon Smith S  |
| CF-L | 20 | Cole Stockton S |
| CF-R | 10 | James Norwood |
| Sub | 17 | Dylan Mottley-Henry |
| Sub | 9 | Paul Mullin |
| Sub | 16 | Mark Ellis |
| Sub | 8 | Jay Harris |
| Sub | 29 | Larnell Cole |
| Sub | 23 | Shamal George |
| Sub | 22 | Harvey Gilmour |
Colchester United | ||
| G | 1 | Dillon Barnes |
| CD-L | 6 | Frankie Kent |
| CD-R | 18 | Tom Eastman |
| LB | 22 | Kane Vincent-Young |
| RB | 2 | Ryan Jackson |
| AM | 9 | Luke Norris S |
| LM | 16 | Diaz Wright |
| RM | 8 | Harry Pell |
| F | 45 | Frank Nouble |
| AM-L | 11 | Brennan Dickenson S |
| AM-R | 10 | Sammie Szmodics |
| Sub | 19 | Mikael Mandron |
| Sub | 7 | Courtney Senior |
| Sub | 39 | Aaron Collins |
| Sub | 23 | Ryan Gondoh |
| Sub | 29 | Ethan Ross |
| Sub | 44 | Ryan Clampin |
| Sub | 35 | Ollie Kensdale |
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