Real Madrid will be without summer signing Arda Guler for Thursday's pre-season friendly against Juventus at the Camping World Stadium in Orlando.
Los Blancos have confirmed that the 18-year-old midfielder – who joined from Fenerbahce earlier this summer – has sustained an injury to his right knee and has returned to Madrid to begin his recovery.
The La Liga giants are also having to cope without fellow midfielder Dani Ceballos, who is recovering from a hamstring injury, while left-back Ferland Mendy is doubtful with a hamstring problem of his own and is unlikely to be risked on Thursday.
Ancelotti has tried and tested a new 4-4-2 diamond formation so far in pre-season, and the Italian boss is expected to continue with the same setup, with big-money signing Jude Bellingham set to start again in the number 10 role.
Federico Valverde, Eduardo Camavinga and Aurelien Tchouameni will also be hoping to retain their places in midfield, but experienced duo Luka Modric and Toni Kroos will be pushing to start after beginning on the bench last time out.
Ancelotti will ponder whether to continue with Vinicius Junior and Rodrygo as two central strikers or recall Brahim Diaz and Joselu to his first XI.
Thibaut Courtois will continue to battle with Andriy Lunin for a start in goal, while the potential absence of Mendy could see David Alaba switch over the left-back and Antonio Rudiger recalled at centre-back to start alongside Eder Militao.
Real Madrid possible starting lineup: Courtois; Carvajal, Militao, Rudiger, Alaba; Kroos; Valverde, Modric; Bellingham; Rodrygo, Vinicius Jr