Real Madrid will be unable to call upon club captain Sergio Ramos in Sunday's key clash with Sevilla.
Ramos, who has struggled with a number of issues in recent months, has not been included in the squad against his former club, having been diagnosed with tendinitis in his left hamstring.
Los Blancos, who are currently second in the La Liga table, two points behind leaders Atletico Madrid, will also definitely be without the services of Ferland Mendy, Lucas Vazquez, Dani Carvajal and Raphael Varane this weekend through injury.
Federico Valverde and Vinicius Junior, like Ramos, missed group training on Friday, but the pair have been named in the squad for the key fixture.
Head coach Zinedine Zidane could be forced to switch to a back four, with Alvaro Odriozola and Marcelo featuring in the full-back positions.
Zidane cannot afford to make wholesale changes from the side that lost 2-0 to Chelsea in the second leg of their Champions League semi-final on Wednesday evening due to the importance of this match.
As a result, Toni Kroos, Casemiro, Luka Modric and Karim Benzema will all start, while there could be a spot in the final third of the field for Marco Asensio, particularly if Vinicius is not deemed fit enough to make the XI.
Eden Hazard's performance against Chelsea and conduct after the match has been heavily criticised, but the Belgium international might be handed another opportunity to prove himself here.
Real Madrid possible starting lineup: Courtois; Odriozola, Militao, Nacho, Marcelo; Modric, Casemiro, Kroos; Hazard, Benzema, Asensio
> Click here to see how Sevilla could line up for this contest
