Alli was injured due to a reckless tackle from Raheem Sterling in Sunday's 2-0 win over Manchester City, but could play.
Stuart Armstrong remains a doubt for Southampton due to a hip problem.
Manager Ralph Hasenhuttl says the midfielder is ahead of schedule in his recovery but the cup replay will probably be too soon.
Provisional Tottenham squad. Lloris, Gazzaniga, Vorm, Aurier, Tanganga, Foyth, Alderweireld, Vertonghen, Sanchez, Sessegnon, Winks, Lo Celso, Ndombele, Alli, Lamela, Moura, Fernandes, Son, Troy Parrott.
Provisional Southampton squad: McCarthy, Bertrand, Vestergaard, Stephens, Bednarek, Ward-Prowse, Hojbjerg, Djenepo, Smallbone, Redmond, Obafemi, Ings, Gunn, Danso, Romeu, Boufal, Long, Adams.