After thrashing Eibar on Saturday afternoon, the Catalan giants watched on as Real Madrid succumbed to a surprise defeat at Levante later on the same day.
Quique Setien's team have now put together a three-match winning streak in Spain's flight, although his players have much to prove on the continent after a number of inconsistent displays during the group stages.
Here, Sports Mole predicts how Barcelona may line up for the fixture at Stadio San Paolo on Tuesday night.
Despite being allowed to sign from Leganes earlier this week, Martin Braithwaite is unable to feature in the Champions League for the rest of the season.
That could see Arturo Vidal remain out on the left flank, a role which he occupied during the five-goal demolition of Eibar at Camp Nou.
As a result of Jordi Alba's adductor injury, Junior Firpo will continue to deputise at left-back, while Sergi Roberto may have been preferred to Nelson Semedo had he not suffered an adductor injury over the weekend.