They will now have to focus on the Champions League, with City already qualified as group winners ahead of this game.
Here, Sports Mole looks at how the Citizens could line up for this fixture.
Guardiola will want to strike the right balance on Wednesday - fielding a team strong enough to win the game while at the same time not risking key players.
On the other side, Guardiola could use this game as a chance to give Zinchenko some match fitness after declaring last week that the player was no longer injured following over a month out.
Ilkay Gundogan was introduced during the closing stages of the Manchester derby and failed to exert an impact, but will be called on once again here as City look to control the tempo early on in the game.
One player who certainly deserves a start, if only to give rest to others, is Phil Foden - a supremely talented youngster but somebody whose minutes have been considerably limited this term.
In a game played at a high tempo, Guardiola may be tempted to pick the killer precision of David Silva, but will be reluctant to use such an important player in a meaningless game.
In the absence of Aguero - another player definitely not being risked in this game - Guardiola will give another chance to Gabriel Jesus, who is yet to start fully firing in front of goal this term.