Carlo Ancelotti has ordered his Real Madrid troops to bounce back from Sunday's 2-1 defeat at Valencia by beating Atletico Madrid in the Copa del Rey.

The Blancos looked good to extend their 22-game winning streak in all competitions after Cristiano Ronaldo fired them into a 14th-minute lead from the penalty spot.

However, second-half goals from Antonio Barragan and Nicolas Otamendi saw Los Che flip the game on its head and deny the visitors their 23rd straight win.

With their first-leg trip to bitter rivals and Spanish champions Atletico coming this Wednesday, the Italian coach is confident that his side will get back to winning ways.

"We have to put the best possible team out to win on Wednesday," he told reporters. "Physically, the team have been good. The season is long and we're well positioned.

"The defeat doesn't affect us. We'll put what we've done so well behind us."

Madrid won the Copa del Rey last season.