Real Madrid left-back Marcelo is confident that Sunday's shock defeat at Valencia will not hinder the Blancos in their first-leg Copa del Rey clash at Atletico Madrid.

Carlo Ancelotti's side travel to the Vicente Calderon on Wednesday on the back of a 2-1 reverse at the Mestalla in a result that ended their 22-game winning streak.

However, Marcelo believes that he and his teammates will regroup in time for part one of their last-16 clash with bitter rivals Atletico.

"I don't think that this defeat will affect the team going into Wednesday," the Brazilian is quoted as saying by Sky Sports News.

"That's another match and we'll be all out to win it. We're going to give our all to win, just like in every game."

Ancelotti and co eliminated the Rojiblancos en route to winning the competition last season.