Spain have revealed that Thiago suffered a knee injury in Friday's international friendly against England.
The Bayern Munich midfielder was forced off in the first period of the clash, which ended in a 2-0 victory for the reigning European champions.
Speculation over the extent of the injury has now been ended, with the Spanish Football Federation confirming that he suffered damage to the ligament in his right knee.
"[Thiago] carried out an MRI scan and results show an edema in the proximal insertion of the medial collateral ligament on his right knee caused by indirect trauma suffered in the match against England," read the statement.
"Taking into account the operations the player has undergone and as a matter of prevention, it has been decided for the player to leave the Spain camp and carry out further tests and work with Bayern Munich's medical staff, who have been in constant contact with Spain's staff since the footballer left the pitch at the Spain-England friendly."
Thiago, who spent the majority of last season on the sidelines with a knee problem, will return to Bayern to continue his treatment and therefore miss Spain's next friendly against Belgium on Tuesday.