Cardiff City forward Lee Tomlin and Accrington Stanley striker Billy Kee have been charged with inflicting grievous bodily harm without intent.
The charge relates to an incident that took place outside a nightclub in Leicester in the early hours of January 22 this year.
Janine Smith, from the Crown Prosecution Service, said in a statement: "The CPS has received a file of evidence from Leicestershire Police relating to an incident in the early hours of January 22.
"Following a review of the evidence, the CPS has authorised the police to charge Lee Tomlin and Billy Kee with inflicting grievous bodily harm without intent.
"The Crown Prosecution Service reminds all concerned that criminal proceedings against Mr Tomlin and Mr Kee are now active and that they have a right to a fair trial.
"It is extremely important that there should be no reporting, commentary or sharing of information online which could in any way prejudice these proceedings."
Tomlin and Kee are due to appear at Leicester Magistrates' Court on September 18.