Zdenek Zeman has dropped Daniele De Rossi, Pablo Daniel Osvaldo and Nicolas Burdisso from his Roma team to face Atalanta BC at the Stadio Olimpico.
Michael Bradley starts his first game since returning from injury, while Leandro Castan, Marquinhos and Ivan Piris form a back four that features four new additions.
Mattia Destro, Francesco Totti and Erik Lamela partner each other in attack at the expense of Osvaldo.
Meanwhile, Atalanta are without eight of their first-team regulars and will also start with a completely untested back line.
Roma: Stekelenburg; Piris, Marquinhos, Castan, Balzaretti; Florenzi, Bradley, Tachtsidis; Destro, Totti, Lamela
Subs: Goicoechea, Svedkauslas, Marquinho, Taddei, Lopez, Perrotta, Burdisso, Romagnoli, Osvaldo, De Rossi
Atalanta: Consigli; Raimondi, Matheu, Peluso, Brivio; Schelotto, Cigarini, Cazzola, Moralez; De Luca, Denis
Subs: Frezzolini, Polito, Ferri, Milesi, Scozzarella, Palma, Bonaventura, Troisi, Marilungo, Parra
