The Brazilian's foot injury rendered him unavailable for the weekend's win over Newcastle United and will keep him sidelined as Liverpool aim to build on a 2-0 first-leg advantage this week.
Naby Keita took his winning goal extremely well at St James' Park, while James Milner produced a man-of-the-match showing, but Fabinho and Thiago Alcantara will be back in the fold this week alongside Jordan Henderson.
Mohamed Salah was also named among the substitutes at the weekend, and the Egypt international will expect to displace Diogo Jota after enjoying a hard-earned rest, with Luis Diaz also holding his place.
Sadio Mane's strike in the first leg was his 14th Champions League knockout goal, and one more here would see him equal the record for goals at this stage of the tournament for an English club - currently held by Frank Lampard.
Liverpool possible starting lineup: Alisson; Alexander-Arnold, Konate, Van Dijk, Robertson; Henderson, Fabinho, Thiago; Salah, Mane, Diaz
> Click here to see how Villarreal could line up for the second leg body check tags ::